# -*-Perl-*- Test Harness script for Bioperl # $Id: TreeIO.t 14580 2008-03-01 17:01:30Z cjfields $ use strict; BEGIN { use lib '.'; use Bio::Root::Test; use File::Temp qw(tempfile); test_begin(-tests => 19); use_ok('Bio::TreeIO'); } my $verbose = 0; #test_debug(); my $treeio = Bio::TreeIO->new( -format => 'nhx', -verbose => $verbose, -file => test_input_file('test.nhx'), ); my $tree; ok($treeio); $tree = $treeio->next_tree; isa_ok($tree, 'Bio::Tree::TreeI'); my @nodes = $tree->get_nodes; is(@nodes, 12, "Total Nodes"); #print STDERR "TREE: ".$tree->as_text('nhx')."\n"; my $adhy = $tree->find_node('ADHY'); is($adhy->branch_length, 0.1); is(($adhy->get_tag_values('S'))[0], 'nematode'); is(($adhy->get_tag_values('E'))[0], '1.1.1.1'); test_roundtrip('((a,b),c);','simple newick'); test_roundtrip('((x:0.05,y:0.06),a:0.1[&&NHX:G=dummy]);','bug 1471 test'); test_roundtrip('((x:0.05[&&NHX:label=x],y:0.06)[&&NHX:label=int_node],a:0.1[&&NHX:label=a]);','different combinations of label, NHX, and branch length'); test_roundtrip('(a:1,b:2,c:3,d:4)TEST:1.2345;','doot node branch length'); test_roundtrip('(A:0.1,B:0.2,(C:0.3,D:0.4)E:0.5)F;','Example from Wikipedia'); test_roundtrip('(((ADH2:0.1[&&NHX:E=1.1.1.1:S=human],ADH1:0.11[&&NHX:E=1.1.1.1:S=human]):0.05[&&NHX:B=100:D=Y:E=1.1.1.1:S=Primates],ADHY:0.1[&&NHX:E=1.1.1.1:S=nematode],ADHX:0.12[&&NHX:E=1.1.1.1:S=insect]):0.1[&&NHX:D=N:E=1.1.1.1:S=Metazoa],(ADH4:0.09[&&NHX:E=1.1.1.1:S=yeast],ADH3:0.13[&&NHX:E=1.1.1.1:S=yeast],ADH2:0.12[&&NHX:E=1.1.1.1:S=yeast],ADH1:0.11[&&NHX:E=1.1.1.1:S=yeast]):0.1[&&NHX:S=Fungi])[&&NHX:D=N:E=1.1.1.1];','ADH NHX tree'); test_roundtrip('(gene1_Hu[&&NHX:S=Hu_Homo_sapiens],(gene2_Hu[&&NHX:S=Hu_Homo_sapiens],gene2_Mu[&&NHX:S=Mu_Mus_musculus]));','notung nhx example http://www.cs.cmu.edu/~aiton/split/Manual-2.6.master014.html'); test_roundtrip('(cow_gene1,(mouse_gene2,cow_gene2)[&&NHX:B=100]);','notung nhx bootstrap http://www.cs.cmu.edu/~aiton/split/Manual-2.6.master014.html'); # Read in some larger trees from data files... test_roundtrip(read_file(test_input_file('nhx-bacteria.nhx')),'r-sig-phylo mailing list http://www.mail-archive.com/r-sig-phylo@r-project.org/msg00516.html'); test_roundtrip(read_file(test_input_file('ex1.nucl.nhx')),'treebest example nhx'); # Note: these files aren't reproduced exactly in their online form. We need to round-trip them once # before including them in the test, because the ordering of annotation keys is not a well-defined # part of the NHX format. Since nhx.pm sorts the keys before output, once they've been through # one time, the ordering becomes stable. test_roundtrip(read_file(test_input_file('wellcome_tol.nhx')),'Wellcome Trust ToL (from http://iphylo.blogspot.com/2009/02/thoughts-on-wellcome-interactive-tree.html)'); # Uncomment to run (takes a long time!!) #test_roundtrip(read_file(test_input_file('tol-2010-02-18.nhx')),'Tolweb.org converted to NHX'); test_roundtrip(read_file(test_input_file('biorecipe.nhx')),'Biorecipes NHX file (http://www.biorecipes.com/Orthologues/StatusPage/pics/TreeEukaryota.nt)'); sub test_roundtrip { my $string = shift; my $desc = shift; my $in = Bio::TreeIO->new(-format => 'nhx', -string => $string, -verbose => $verbose ); my $t = $in->next_tree; my $out; if (defined $t) { $out = $t->as_text('nhx'); } $desc = "Roundtrip: $desc"; return is($out,$string,$desc); } sub read_file { my $file = shift; local $/=undef; my $string; open IN, $file or die ("Couldn't open file [$file]: $!"); binmode IN; $string = ; close IN; $string =~ s/\n//g; #print STDERR "STR: $string\n"; return $string; }