# $Id: Node.pm 844 2009-03-05 00:07:26Z rvos $ package Bio::Phylo::Forest::Node; use strict; use Bio::Phylo::Taxa::TaxonLinker; use Bio::Phylo::Util::CONSTANT qw(_NODE_ _TREE_ _TAXON_ looks_like_number looks_like_object looks_like_hash); use Bio::Phylo::Listable; use Bio::Phylo::Util::Exceptions 'throw'; use Scalar::Util 'weaken'; no warnings 'recursion'; # classic @ISA manipulation, not using 'base' use vars qw(@ISA); @ISA = qw( Bio::Phylo::Taxa::TaxonLinker Bio::Phylo::Listable ); my $LOADED_WRAPPERS = 0; { # logger singleton my $logger = __PACKAGE__->get_logger; # store type constant my ( $TYPE_CONSTANT, $CONTAINER_CONSTANT ) = ( _NODE_, _TREE_ ); # @fields array necessary for object destruction my @fields = \( my ( %branch_length, %parent, %tree ) ); =head1 NAME Bio::Phylo::Forest::Node - Node in a phylogenetic tree =head1 SYNOPSIS # some way to get nodes: use Bio::Phylo::IO; my $string = '((A,B),C);'; my $forest = Bio::Phylo::IO->parse( -format => 'newick', -string => $string ); # prints 'Bio::Phylo::Forest' print ref $forest; foreach my $tree ( @{ $forest->get_entities } ) { # prints 'Bio::Phylo::Forest::Tree' print ref $tree; foreach my $node ( @{ $tree->get_entities } ) { # prints 'Bio::Phylo::Forest::Node' print ref $node; # node has a parent, i.e. is not root if ( $node->get_parent ) { $node->set_branch_length(1); } # node is root else { $node->set_branch_length(0); } } } =head1 DESCRIPTION This module defines a node object and its methods. The node is fairly syntactically rich in terms of navigation, and additional getters are provided to further ease navigation from node to node. Typical first daughter -> next sister traversal and recursion is possible, but there are also shrinkwrapped methods that return for example all terminal descendants of the focal node, or all internals, etc. Node objects are inserted into tree objects, although technically the tree object is only a container holding all the nodes together. Unless there are orphans all nodes can be reached without recourse to the tree object. =head1 METHODS =head2 CONSTRUCTOR =over =item new() Node constructor. Type : Constructor Title : new Usage : my $node = Bio::Phylo::Forest::Node->new; Function: Instantiates a Bio::Phylo::Forest::Node object Returns : Bio::Phylo::Forest::Node Args : All optional: -parent => $parent, -taxon => $taxon, -branch_length => 0.423e+2, -first_daughter => $f_daughter, -last_daughter => $l_daughter, -next_sister => $n_sister, -previous_sister => $p_sister, -name => 'node_name', -desc => 'this is a node', -score => 0.98, -generic => { -posterior => 0.98, -bootstrap => 0.80 } =cut sub new { # could be child class my $class = shift; # notify user $logger->info("constructor called for '$class'"); # process bioperl args my %args = looks_like_hash @_; if ( exists $args{'-leaf'} ) { delete $args{'-leaf'}; } if ( exists $args{'-id'} ) { my $name = $args{'-id'}; delete $args{'-id'}; $args{'-name'} = $name; } if ( exists $args{'-nhx'} ) { my $hash = $args{'-nhx'}; delete $args{'-nhx'}; $args{'-generic'} = $hash; } if ( not exists $args{'-tag'} ) { $args{'-tag'} = 'node'; } # go up inheritance tree, eventually get an ID my $self = $class->SUPER::new(%args); if ( not $LOADED_WRAPPERS ) { eval do { local $/; }; $LOADED_WRAPPERS++; } return $self; } my $set_raw_parent = sub { my ( $self, $parent ) = @_; $parent{ $$self } = $parent; # XXX here we modify parent weaken $parent{ $$self } if $parent; }; my $get_parent = sub { my $self = shift; return $parent{ $$self }; }; my $get_children = sub { shift->get_entities }; my $get_branch_length = sub { my $self = shift; return $branch_length{ $$self }; }; my $set_raw_child = sub { my ( $self, $child, $i ) = @_; $i = $self->last_index + 1 if not defined $i or $i == -1; $self->insert_at_index( $child, $i ); # XXX here we modify children }; =item new_from_bioperl() Node constructor from bioperl L argument. Type : Constructor Title : new_from_bioperl Usage : my $node = Bio::Phylo::Forest::Node->new_from_bioperl( $bpnode ); Function: Instantiates a Bio::Phylo::Forest::Node object from a bioperl node object. Returns : Bio::Phylo::Forest::Node Args : An objects that implements Bio::Tree::NodeI Notes : The following BioPerl properties are copied: BioPerl output: Bio::Phylo output: ------------------------------------------------ id get_name branch_length get_branch_length description get_desc bootstrap get_generic('bootstrap') In addition all BioPerl tags and values are copied to set_generic( 'tag' => 'value' ); =cut sub new_from_bioperl { my ( $class, $bpnode ) = @_; my $node = $class->new; # copy name my $name = $bpnode->id; $node->set_name( $name ) if defined $name; # copy branch length my $branch_length = $bpnode->branch_length; $node->set_branch_length( $branch_length ) if defined $branch_length; # copy description my $desc = $bpnode->description; $node->set_desc( $desc ) if defined $desc; # copy bootstrap my $bootstrap = $bpnode->bootstrap; $node->set_score( $bootstrap ) if defined $bootstrap and looks_like_number $bootstrap; # copy other tags for my $tag ( $bpnode->get_all_tags ) { my @values = $bpnode->get_tag_values( $tag ); $node->set_generic( $tag => \@values ); } return $node; } =back =head2 MUTATORS =over =item prune_child() Sets argument as invocant's parent. Type : Mutator Title : prune_child Usage : $parent->prune_child($child); Function: Removes $child (and its descendants) from $parent's children Returns : Modified object. Args : A valid argument is Bio::Phylo::Forest::Node object. =cut sub prune_child { my ( $self, $child ) = @_; $self->delete( $child ); return $self; } =item collapse() Collapse node. Type : Mutator Title : collapse Usage : $node->collapse; Function: Attaches invocant's children to invocant's parent. Returns : Modified object. Args : NONE Comments: If defined, adds invocant's branch length to that of its children. If $node is in a tree, removes itself from that tree. =cut sub collapse { my $self = shift; # can't collapse root if ( my $parent = $self->get_parent ) { # can't collapse terminal nodes if ( my @children = @{ $self->get_children } ) { # add node's branch length to that of children my $length = $self->get_branch_length; for my $child ( @children ) { if ( defined $length ) { my $child_length = $child->get_branch_length || 0; $child->set_branch_length( $length + $child_length ); } # attach children to node's parent $child->set_parent( $parent ); } # prune node from parent $parent->prune_child( $self ); # delete node from tree if ( my $tree = $self->_get_container ) { $tree->delete( $self ); } } else { return $self; } } else { return $self; } } =item set_parent() Sets argument as invocant's parent. Type : Mutator Title : set_parent Usage : $node->set_parent($parent); Function: Assigns a node's parent. Returns : Modified object. Args : If no argument is given, the current parent is set to undefined. A valid argument is Bio::Phylo::Forest::Node object. =cut sub set_parent { my ( $self, $parent ) = @_; if ( $parent and looks_like_object $parent, $TYPE_CONSTANT ) { $parent->set_child( $self ); } elsif ( not $parent ) { $set_raw_parent->( $self ); } return $self; } =item set_first_daughter() Sets argument as invocant's first daughter. Type : Mutator Title : set_first_daughter Usage : $node->set_first_daughter($f_daughter); Function: Assigns a node's leftmost daughter. Returns : Modified object. Args : Undefines the first daughter if no argument given. A valid argument is a Bio::Phylo::Forest::Node object. =cut sub set_first_daughter { my ( $self, $fd ) = @_; $self->set_child( $fd, 0 ); return $self; } =item set_last_daughter() Sets argument as invocant's last daughter. Type : Mutator Title : set_last_daughter Usage : $node->set_last_daughter($l_daughter); Function: Assigns a node's rightmost daughter. Returns : Modified object. Args : A valid argument consists of a Bio::Phylo::Forest::Node object. If no argument is given, the value is set to undefined. =cut sub set_last_daughter { my ( $self, $ld ) = @_; $self->set_child( $ld, scalar @{ $self->get_children } ); return $self; } =item set_previous_sister() Sets argument as invocant's previous sister. Type : Mutator Title : set_previous_sister Usage : $node->set_previous_sister($p_sister); Function: Assigns a node's previous sister (to the left). Returns : Modified object. Args : A valid argument consists of a Bio::Phylo::Forest::Node object. If no argument is given, the value is set to undefined. =cut sub set_previous_sister { my ( $self, $ps ) = @_; if ( $ps and looks_like_object $ps, $TYPE_CONSTANT ) { if ( my $parent = $self->get_parent ) { my $children = $parent->get_children; my $j = 0; FINDSELF: for ( my $i = $#{ $children }; $i >= 0; $i-- ) { if ( $children->[$i] == $self ) { $j = $i - 1; last FINDSELF; } } $j = 0 if $j == -1; $parent->set_child( $ps, $j ); } } return $self; } =item set_next_sister() Sets argument as invocant's next sister. Type : Mutator Title : set_next_sister Usage : $node->set_next_sister($n_sister); Function: Assigns or retrieves a node's next sister (to the right). Returns : Modified object. Args : A valid argument consists of a Bio::Phylo::Forest::Node object. If no argument is given, the value is set to undefined. =cut sub set_next_sister { my ( $self, $ns ) = @_; if ( $ns and looks_like_object $ns, $TYPE_CONSTANT ) { if ( my $parent = $self->get_parent ) { my $children = $parent->get_children; my $last = scalar @{ $children }; my $j = $last; FINDSELF: for my $i ( 0 .. $#{ $children } ) { if ( $children->[$i] == $self ) { $j = $i + 1; last FINDSELF; } } $parent->set_child( $ns, $j ); } } return $self; } =item set_child() Sets argument as invocant's child. Type : Mutator Title : set_child Usage : $node->set_child($child); Function: Assigns a new child to $node Returns : Modified object. Args : A valid argument consists of a Bio::Phylo::Forest::Node object. =cut sub set_child { my ( $self, $child, $i ) = @_; # bad args? if ( not $child or not looks_like_object $child, $TYPE_CONSTANT ) { return; } # maybe nothing to do? if ( not $child or $child->get_id == $self->get_id or $child->is_child_of($self) ) { return $self; } # $child_parent is NEVER $self, see above my $child_parent = $child->get_parent; # child is ancestor: this is obviously problematic, because # now we're trying to set a node nearer to the root on the # same lineage as the CHILD of a descendant. Because they're # on the same lineage it's hard to see how this can be done # sensibly. The decision here is to do: # 1. we prune what is to become the parent (now the descendant) # from its current parent # 2. we set this pruned node (and its descendants) as a sibling # of what is to become the child # 3. we prune what is to become the child from its parent # 4. we set that pruned child as the child of $self if ( $child->is_ancestor_of( $self ) ) { # step 1. my $parent_parent = $self->get_parent; $parent_parent->prune_child( $self ); # step 2. $set_raw_parent->( $self, $child_parent ); # XXX could be undef if ( $child_parent ) { $set_raw_child->( $child_parent, $self ); } } # step 3. if ( $child_parent ) { $child_parent->prune_child( $child ); } $set_raw_parent->( $child, $self ); # now do the insert, first make room by shifting later siblings right my $children = $self->get_children; if ( defined $i ) { for ( my $j = $#{ $children }; $j >= 0; $j-- ) { my $sibling = $children->[$j]; $set_raw_child->( $self, $sibling, $j + 1 ); } } # no index was supplied, child becomes last daughter else { $i = scalar @{ $children }; } # step 4. $set_raw_child->( $self, $child, $i ); return $self; } =item set_branch_length() Sets argument as invocant's branch length. Type : Mutator Title : set_branch_length Usage : $node->set_branch_length(0.423e+2); Function: Assigns a node's branch length. Returns : Modified object. Args : If no argument is given, the current branch length is set to undefined. A valid argument is a number in any of Perl's formats. =cut sub set_branch_length { my ( $self, $bl ) = @_; my $id = $$self; if ( defined $bl && looks_like_number $bl && !ref $bl ) { $branch_length{$id} = $bl; } elsif ( defined $bl && ( !looks_like_number $bl || ref $bl ) ) { throw 'BadNumber' => "Branch length \"$bl\" is a bad number"; } elsif ( !defined $bl ) { $branch_length{$id} = undef; } return $self; } =item set_node_below() Sets new (unbranched) node below invocant. Type : Mutator Title : set_node_below Usage : my $new_node = $node->set_node_below; Function: Creates a new node below $node Returns : New node if tree was modified, undef otherwise Args : NONE =cut sub set_node_below { my $self = shift; # can't set node below root if ( $self->is_root ) { return; } # instantiate new node from $self's class my $new_node = ( ref $self )->new( @_ ); # attach new node to $child's parent my $parent = $self->get_parent; $parent->set_child( $new_node ); # insert new node in tree # if ( my $tree = $self->_get_container ) { # $tree->insert( $new_node ); # } # attach $self to new node $new_node->set_child( $self ); # done return $new_node; } =item set_root_below() Reroots below invocant. Type : Mutator Title : set_root_below Usage : $node->set_root_below; Function: Creates a new tree root below $node Returns : New root if tree was modified, undef otherwise Args : NONE Comments: Implementation incomplete: returns spurious results when $node is grandchild of current root. =cut # Example tree to illustrate rerooting algorithm: # # A B C D E F # \ / / / / / # \_/ / / / / # 1 / / / / # new->\ / / / / # \_/ / / / # 2 / / / # \ / / / # \_/ / / # 3 / / # \ / / # \_/ / # 4 / # \ / # \_/ # 5 # | sub set_root_below { my $self = shift; my %constructor_args = @_; $constructor_args{'-name'} = 'root' if not $constructor_args{'-name'}; # $self is node 5, nothing to do, # can't place root below root if ( $self->is_root ) { return; } my @ancestors = @{ $self->get_ancestors }; # if @ancestors = ( 5 ); i.e. $self is node 4 # root is already below $self if ( scalar @ancestors == 1 ) { return; } # let's say $self is node 1, ancestors is: # ( 2, 3, 4, 5 ) -> ( 2, 3, 4 ) my $root = pop @ancestors; # ( 2, 3, 4 ) -> ( 2, 3 ) my $node_above_root = pop @ancestors; # collapse node 4 $node_above_root->collapse; # ( 2, 3 ) -> ( 2, 3, 5 ); 4 doesn't exist anymore (collapsed) push @ancestors, $root; # ( 2, 3, 5 ) -> ( new, 2, 3, 5 ) unshift @ancestors, $self->set_node_below(%constructor_args); # i.e. $self wasn't 3 if ( scalar @ancestors > 2 ) { for ( my $i = $#ancestors; $i >= 0; $i-- ) { # flip parent & child $ancestors[$i]->set_child( $ancestors[$i+1] ); } } else { # XXX $logger->warn; $ancestors[0]->set_child( $ancestors[1] ); } if ( my $tree = $self->get_tree ) { $tree->insert($ancestors[0]); } return $ancestors[0]; } # sub set_root_below { # my $node = shift; # if ( $node->get_ancestors ) { # my @ancestors = @{ $node->get_ancestors }; # # # first collapse root # my $root = $ancestors[-1]; # my $lineage_containing_node; # my @children = @{ $root->get_children }; # FIND_LINEAGE: for my $child (@children) { # if ( $child->get_id == $node->get_id ) { # $lineage_containing_node = $child; # last FIND_LINEAGE; # } # for my $descendant ( @{ $child->get_descendants } ) { # if ( $descendant->get_id == $node->get_id ) { # $lineage_containing_node = $child; # last FIND_LINEAGE; # } # } # } # for my $child (@children) { # next if $child->get_id == $lineage_containing_node->get_id; # $child->set_parent($lineage_containing_node); # } # # # now create new root as parent of $node # my $newroot = __PACKAGE__->new( '-name' => 'root' ); # $node->set_parent($newroot); # # # update list of ancestors, want to get rid of old root # # at $ancestors[-1] and have new root as $ancestors[0] # unshift @ancestors, $newroot; # pop @ancestors; # # # update connections # for ( my $i = $#ancestors ; $i >= 1 ; $i-- ) { # $ancestors[$i]->set_parent( $ancestors[ $i - 1 ] ); # } # # # delete root if part of tree, insert new # if ( my $tree = $node->_get_container ) { # $tree->delete($root); # $tree->insert($newroot); # } # } # } =item set_tree() Sets what tree invocant belongs to Type : Mutator Title : set_tree Usage : $node->set_tree($tree); Function: Sets what tree invocant belongs to Returns : Invocant Args : Bio::Phylo::Forest::Tree Comments: This method is called automatically when inserting or deleting nodes in trees. =cut sub set_tree { my ( $self, $tree ) = @_; my $id = $self->get_id; if ( $tree ) { if ( looks_like_object $tree, $CONTAINER_CONSTANT ) { $tree{$id} = $tree; weaken $tree{$id}; } else { throw 'ObjectMismatch' => "$tree is not a tree"; } } else { $tree{$id} = undef; } return $self; } =back =head2 ACCESSORS =over =item get_parent() Gets invocant's parent. Type : Accessor Title : get_parent Usage : my $parent = $node->get_parent; Function: Retrieves a node's parent. Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_parent { return $get_parent->( shift ) } =item get_first_daughter() Gets invocant's first daughter. Type : Accessor Title : get_first_daughter Usage : my $f_daughter = $node->get_first_daughter; Function: Retrieves a node's leftmost daughter. Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_first_daughter { return $_[0]->get_child(0); } =item get_last_daughter() Gets invocant's last daughter. Type : Accessor Title : get_last_daughter Usage : my $l_daughter = $node->get_last_daughter; Function: Retrieves a node's rightmost daughter. Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_last_daughter { return $_[0]->get_child(-1); } =item get_previous_sister() Gets invocant's previous sister. Type : Accessor Title : get_previous_sister Usage : my $p_sister = $node->get_previous_sister; Function: Retrieves a node's previous sister (to the left). Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_previous_sister { my ( $self ) = @_; my $ps; if ( my $parent = $self->get_parent ) { my $children = $parent->get_children; FINDSELF: for ( my $i = $#{ $children }; $i >= 1; $i-- ) { if ( $children->[$i] == $self ) { $ps = $children->[$i - 1]; last FINDSELF; } } } return $ps; } =item get_next_sister() Gets invocant's next sister. Type : Accessor Title : get_next_sister Usage : my $n_sister = $node->get_next_sister; Function: Retrieves a node's next sister (to the right). Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_next_sister { my ( $self ) = @_; my $ns; if ( my $parent = $self->get_parent ) { my $children = $parent->get_children; FINDSELF: for my $i ( 0 .. $#{ $children } ) { if ( $children->[$i] == $self ) { $ns = $children->[$i + 1]; last FINDSELF; } } } return $ns; } =item get_branch_length() Gets invocant's branch length. Type : Accessor Title : get_branch_length Usage : my $branch_length = $node->get_branch_length; Function: Retrieves a node's branch length. Returns : FLOAT Args : NONE Comments: Test for "defined($node->get_branch_length)" for zero-length (but defined) branches. Testing "if ( $node->get_branch_length ) { ... }" yields false for zero-but-defined branches! =cut sub get_branch_length { return $get_branch_length->( shift ) } =item get_ancestors() Gets invocant's ancestors. Type : Query Title : get_ancestors Usage : my @ancestors = @{ $node->get_ancestors }; Function: Returns an array reference of ancestral nodes, ordered from young to old (i.e. $ancestors[-1] is root). Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : NONE =cut sub get_ancestors { my $self = shift; my @ancestors; my $node = $self; if ( $node = $node->get_parent ) { while ($node) { push @ancestors, $node; $node = $node->get_parent; } return \@ancestors; } else { return; } } =item get_sisters() Gets invocant's sisters. Type : Query Title : get_sisters Usage : my @sisters = @{ $node->get_sisters }; Function: Returns an array reference of sisters, ordered from left to right. Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : NONE =cut sub get_sisters { my $self = shift; my $sisters = $self->get_parent->get_children; return $sisters; } =item get_children() Gets invocant's immediate children. Type : Query Title : get_children Usage : my @children = @{ $node->get_children }; Function: Returns an array reference of immediate descendants, ordered from left to right. Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : NONE =cut sub get_children { return $get_children->( shift ) } =item get_child() Gets invocant's i'th child. Type : Query Title : get_child Usage : my $child = $node->get_child($i); Function: Returns the child at index $i Returns : A Bio::Phylo::Forest::Node object. Args : An index (integer) $i Comments: if no index is specified, first child is returned =cut sub get_child { my ( $self, $i ) = @_; $i = 0 if not defined $i; my $children = $self->get_children; return $children->[$i]; } =item get_descendants() Gets invocant's descendants. Type : Query Title : get_descendants Usage : my @descendants = @{ $node->get_descendants }; Function: Returns an array reference of descendants, recursively ordered breadth first. Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : none. =cut sub get_descendants { my $self = shift; my @current = ($self); my @desc; while ( $self->_desc(@current) ) { @current = $self->_desc(@current); push @desc, @current; } return \@desc; } =begin comment Type : Internal method Title : _desc Usage : $node->_desc(\@nodes); Function: Performs recursion for Bio::Phylo::Forest::Node::get_descendants() Returns : A Bio::Phylo::Forest::Node object. Args : A Bio::Phylo::Forest::Node object. Comments: This method works in conjunction with Bio::Phylo::Forest::Node::get_descendants() - the latter simply calls the former with a set of nodes, and the former returns their children. Bio::Phylo::Forest::Node::get_descendants() then calls Bio::Phylo::Forest::Node::_desc with this set of children, and so on until all nodes are terminals. A first_daughter -> next_sister postorder traversal in a single method would have been more elegant - though not more efficient, in terms of visited nodes. =end comment =cut sub _desc { my $self = shift; my @current = @_; my @return; foreach (@current) { my $children = $_->get_children; if ($children) { push @return, @{$children}; } } return @return; } =item get_terminals() Gets invocant's terminal descendants. Type : Query Title : get_terminals Usage : my @terminals = @{ $node->get_terminals }; Function: Returns an array reference of terminal descendants. Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : NONE =cut sub get_terminals { my $self = shift; my @terminals; my $desc = $self->get_descendants; if ( @{$desc} ) { foreach ( @{$desc} ) { if ( $_->is_terminal ) { push @terminals, $_; } } } return \@terminals; } =item get_internals() Gets invocant's internal descendants. Type : Query Title : get_internals Usage : my @internals = @{ $node->get_internals }; Function: Returns an array reference of internal descendants. Returns : Array reference of Bio::Phylo::Forest::Node objects. Args : NONE =cut sub get_internals { my $self = shift; my @internals; my $desc = $self->get_descendants; if ( @{$desc} ) { foreach ( @{$desc} ) { if ( $_->is_internal ) { push @internals, $_; } } } return \@internals; } =item get_mrca() Gets invocant's most recent common ancestor shared with argument. Type : Query Title : get_mrca Usage : my $mrca = $node->get_mrca($other_node); Function: Returns the most recent common ancestor of $node and $other_node. Returns : Bio::Phylo::Forest::Node Args : A Bio::Phylo::Forest::Node object in the same tree. =cut sub get_mrca { my ( $self, $other_node ) = @_; my $self_anc = $self->get_ancestors; my $other_anc = $other_node->get_ancestors; for my $i ( 0 .. $#{$self_anc} ) { for my $j ( 0 .. $#{$other_anc} ) { if ( ${ $self_anc->[$i] } == ${ $other_anc->[$j] } ) { return $self_anc->[$i]; } } } $logger->warn( "using " . $self_anc->[-1]->get_internal_name ); return $self_anc->[-1]; } =item get_leftmost_terminal() Gets invocant's leftmost terminal descendant. Type : Query Title : get_leftmost_terminal Usage : my $leftmost_terminal = $node->get_leftmost_terminal; Function: Returns the leftmost terminal descendant of $node. Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_leftmost_terminal { my $self = shift; my $daughter = $self; FIRST_DAUGHTER: while ($daughter) { if ( my $grand_daughter = $daughter->get_first_daughter ) { $daughter = $grand_daughter; next FIRST_DAUGHTER; } else { last FIRST_DAUGHTER; } } return $daughter; } =item get_rightmost_terminal() Gets invocant's rightmost terminal descendant Type : Query Title : get_rightmost_terminal Usage : my $rightmost_terminal = $node->get_rightmost_terminal; Function: Returns the rightmost terminal descendant of $node. Returns : Bio::Phylo::Forest::Node Args : NONE =cut sub get_rightmost_terminal { my $self = shift; my $daughter = $self; LAST_DAUGHTER: while ($daughter) { if ( my $grand_daughter = $daughter->get_last_daughter ) { $daughter = $grand_daughter; next LAST_DAUGHTER; } else { last LAST_DAUGHTER; } } return $daughter; } =item get_tree() Returns the tree invocant belongs to Type : Query Title : get_tree Usage : my $tree = $node->get_tree; Function: Returns the tree $node belongs to Returns : Bio::Phylo::Forest::Tree Args : NONE =cut sub get_tree { my $self = shift; my $id = $self->get_id; return $tree{$id}; } =back =head2 TESTS =over =item is_terminal() Tests if invocant is a terminal node. Type : Test Title : is_terminal Usage : if ( $node->is_terminal ) { # do something } Function: Returns true if node has no children (i.e. is terminal). Returns : BOOLEAN Args : NONE =cut sub is_terminal { return !shift->get_first_daughter; } =item is_internal() Tests if invocant is an internal node. Type : Test Title : is_internal Usage : if ( $node->is_internal ) { # do something } Function: Returns true if node has children (i.e. is internal). Returns : BOOLEAN Args : NONE =cut sub is_internal { return !!shift->get_first_daughter; } =item is_first() Tests if invocant is first sibling in left-to-right order. Type : Test Title : is_first Usage : if ( $node->is_first ) { # do something } Function: Returns true if first sibling in left-to-right order. Returns : BOOLEAN Args : NONE =cut sub is_first { return !shift->get_previous_sister; } =item is_last() Tests if invocant is last sibling in left-to-right order. Type : Test Title : is_last Usage : if ( $node->is_last ) { # do something } Function: Returns true if last sibling in left-to-right order. Returns : BOOLEAN Args : NONE =cut sub is_last { return !shift->get_next_sister; } =item is_root() Tests if invocant is a root. Type : Test Title : is_root Usage : if ( $node->is_root ) { # do something } Function: Returns true if node is a root Returns : BOOLEAN Args : NONE =cut sub is_root { return !shift->get_parent; } =item is_descendant_of() Tests if invocant is descendant of argument. Type : Test Title : is_descendant_of Usage : if ( $node->is_descendant_of($grandparent) ) { # do something } Function: Returns true if the node is a descendant of the argument. Returns : BOOLEAN Args : putative ancestor - a Bio::Phylo::Forest::Node object. =cut sub is_descendant_of { my ( $self, $ancestor ) = @_; my $ancestor_id = $$ancestor; while ($self) { if ( my $parent = $self->get_parent ) { $self = $parent; } else { return; } if ( $$self == $ancestor_id ) { return 1; } } } =item is_ancestor_of() Tests if invocant is ancestor of argument. Type : Test Title : is_ancestor_of Usage : if ( $node->is_ancestor_of($grandchild) ) { # do something } Function: Returns true if the node is an ancestor of the argument. Returns : BOOLEAN Args : putative descendant - a Bio::Phylo::Forest::Node object. =cut sub is_ancestor_of { my ( $self, $child ) = @_; if ( $child->is_descendant_of($self) ) { return 1; } else { return; } } =item is_sister_of() Tests if invocant is sister of argument. Type : Test Title : is_sister_of Usage : if ( $node->is_sister_of($sister) ) { # do something } Function: Returns true if the node is a sister of the argument. Returns : BOOLEAN Args : putative sister - a Bio::Phylo::Forest::Node object. =cut sub is_sister_of { my ( $self, $sister ) = @_; my ( $self_parent, $sister_parent ) = ( $self->get_parent, $sister->get_parent ); if ( $self_parent && $sister_parent && $self_parent->get_id == $sister_parent->get_id ) { return 1; } else { return; } } =item is_child_of() Tests if invocant is child of argument. Type : Test Title : is_child_of Usage : if ( $node->is_child_of($parent) ) { # do something } Function: Returns true if the node is a child of the argument. Returns : BOOLEAN Args : putative parent - a Bio::Phylo::Forest::Node object. =cut sub is_child_of { my ( $self, $node ) = @_; if ( my $parent = $self->get_parent ) { return $parent->get_id == $node->get_id; } return 0; } =item is_outgroup_of() Test if invocant is outgroup of argument nodes. Type : Test Title : is_outgroup_of Usage : if ( $node->is_outgroup_of(\@ingroup) ) { # do something } Function: Tests whether the set of \@ingroup is monophyletic with respect to the $node. Returns : BOOLEAN Args : A reference to an array of Bio::Phylo::Forest::Node objects; Comments: This method is essentially the same as &Bio::Phylo::Forest::Tree::is_monophyletic. =cut sub is_outgroup_of { my ( $outgroup, $nodes ) = @_; for my $i ( 0 .. $#{$nodes} ) { for my $j ( ( $i + 1 ) .. $#{$nodes} ) { my $mrca = $nodes->[$i]->get_mrca( $nodes->[$j] ); return if $mrca->is_ancestor_of($outgroup); } } return 1; } =item can_contain() Test if argument(s) can be a child/children of invocant. Type : Test Title : can_contain Usage : if ( $parent->can_contain(@children) ) { # do something } Function: Test if arguments can be children of invocant. Returns : BOOLEAN Args : An array of Bio::Phylo::Forest::Node objects; Comments: This method is an override of Bio::Phylo::Listable::can_contain. Since node objects hold a list of their children, they inherit from the listable class and so they need to be able to validate the contents of that list before they are inserted. =cut sub can_contain { my $self = shift; my $type = $self->_type; for ( @_ ) { return 0 if $type != $_->_type; } return 1; } =back =head2 CALCULATIONS =over =item calc_path_to_root() Calculates path to root. Type : Calculation Title : calc_path_to_root Usage : my $path_to_root = $node->calc_path_to_root; Function: Returns the sum of branch lengths from $node to the root. Returns : FLOAT Args : NONE =cut sub calc_path_to_root { my $self = shift; my $node = $self; my $path = 0; while ($node) { my $branch_length = $node->get_branch_length; if ( defined $branch_length ) { $path += $branch_length; } if ( my $parent = $node->get_parent ) { $node = $parent; } else { last; } } return $path; } =item calc_nodes_to_root() Calculates number of nodes to root. Type : Calculation Title : calc_nodes_to_root Usage : my $nodes_to_root = $node->calc_nodes_to_root; Function: Returns the number of nodes from $node to the root. Returns : INT Args : NONE =cut sub calc_nodes_to_root { my $self = shift; my ( $nodes, $parent ) = ( 0, $self ); while ($parent) { $nodes++; $parent = $parent->get_parent; if ($parent) { if ( my $cntr = $parent->calc_nodes_to_root ) { $nodes += $cntr; last; } } } return $nodes; } =item calc_max_nodes_to_tips() Calculates maximum number of nodes to tips. Type : Calculation Title : calc_max_nodes_to_tips Usage : my $max_nodes_to_tips = $node->calc_max_nodes_to_tips; Function: Returns the maximum number of nodes from $node to tips. Returns : INT Args : NONE =cut sub calc_max_nodes_to_tips { my $self = shift; my $self_id = $self->get_id; my ( $nodes, $maxnodes ) = ( 0, 0 ); foreach my $child ( @{ $self->get_terminals } ) { $nodes = 0; while ( $child && $child->get_id != $self_id ) { $nodes++; $child = $child->get_parent; } if ( $nodes > $maxnodes ) { $maxnodes = $nodes; } } return $maxnodes; } =item calc_min_nodes_to_tips() Calculates minimum number of nodes to tips. Type : Calculation Title : calc_min_nodes_to_tips Usage : my $min_nodes_to_tips = $node->calc_min_nodes_to_tips; Function: Returns the minimum number of nodes from $node to tips. Returns : INT Args : NONE =cut sub calc_min_nodes_to_tips { my $self = shift; my $self_id = $self->get_id; my ( $nodes, $minnodes ); foreach my $child ( @{ $self->get_terminals } ) { $nodes = 0; while ( $child && $child->get_id != $self_id ) { $nodes++; $child = $child->get_parent; } if ( !$minnodes || $nodes < $minnodes ) { $minnodes = $nodes; } } return $minnodes; } =item calc_max_path_to_tips() Calculates longest path to tips. Type : Calculation Title : calc_max_path_to_tips Usage : my $max_path_to_tips = $node->calc_max_path_to_tips; Function: Returns the path length from $node to the tallest tip. Returns : FLOAT Args : NONE =cut sub calc_max_path_to_tips { my $self = shift; my $id = $self->get_id; my ( $length, $maxlength ) = ( 0, 0 ); foreach my $child ( @{ $self->get_terminals } ) { $length = 0; while ( $child && $child->get_id != $id ) { my $branch_length = $child->get_branch_length; if ( defined $branch_length ) { $length += $branch_length; } $child = $child->get_parent; } if ( $length > $maxlength ) { $maxlength = $length; } } return $maxlength; } =item calc_min_path_to_tips() Calculates shortest path to tips. Type : Calculation Title : calc_min_path_to_tips Usage : my $min_path_to_tips = $node->calc_min_path_to_tips; Function: Returns the path length from $node to the shortest tip. Returns : FLOAT Args : NONE =cut sub calc_min_path_to_tips { my $self = shift; my $id = $self->get_id; my ( $length, $minlength ); foreach my $child ( @{ $self->get_terminals } ) { $length = 0; while ( $child && $child->get_id != $id ) { my $branch_length = $child->get_branch_length; if ( defined $branch_length ) { $length += $branch_length; } $child = $child->get_parent; } if ( !$minlength ) { $minlength = $length; } if ( $length < $minlength ) { $minlength = $length; } } return $minlength; } =item calc_patristic_distance() Calculates patristic distance between invocant and argument. Type : Calculation Title : calc_patristic_distance Usage : my $patristic_distance = $node->calc_patristic_distance($other_node); Function: Returns the patristic distance between $node and $other_node. Returns : FLOAT Args : Bio::Phylo::Forest::Node =cut sub calc_patristic_distance { my ( $self, $other_node ) = @_; my $patristic_distance; my $mrca = $self->get_mrca($other_node); my $mrca_id = $mrca->get_id; while ( $self->get_id != $mrca_id ) { my $branch_length = $self->get_branch_length; if ( defined $branch_length ) { $patristic_distance += $branch_length; } $self = $self->get_parent; } while ( $other_node and $other_node->get_id != $mrca_id ) { my $branch_length = $other_node->get_branch_length; if ( defined $branch_length ) { $patristic_distance += $branch_length; } $other_node = $other_node->get_parent; } return $patristic_distance; } =item calc_nodal_distance() Calculates node distance between invocant and argument. Type : Calculation Title : calc_nodal_distance Usage : my $nodal_distance = $node->calc_nodal_distance($other_node); Function: Returns the number of nodes between $node and $other_node. Returns : INT Args : Bio::Phylo::Forest::Node =cut sub calc_nodal_distance { my ( $self, $other_node ) = @_; my $nodal_distance; my $mrca = $self->get_mrca( $other_node ); my $mrca_id = $mrca->get_id; while ( $self and $self->get_id != $mrca_id ) { $nodal_distance++; $self = $self->get_parent; } while ( $other_node and $other_node->get_id != $mrca_id ) { $nodal_distance++; $other_node = $other_node->get_parent; } return $nodal_distance; } =back =head2 VISITOR METHODS The methods below are similar in spirit to those by the same name in L, except those in the tree class operate from the tree root, and those in this node class operate on an invocant node, and so these process a subtree. =over =item visit_depth_first() Visits nodes depth first Type : Visitor method Title : visit_depth_first Usage : $tree->visit_depth_first( -pre => sub{ ... }, -post => sub { ... } ); Function: Visits nodes in a depth first traversal, executes subs Returns : $tree Args : Optional: # first event handler, is executed when node is reached in recursion -pre => sub { print "pre: ", shift->get_name, "\n" }, # is executed if node has a daughter, but before that daughter is processed -pre_daughter => sub { print "pre_daughter: ", shift->get_name, "\n" }, # is executed if node has a daughter, after daughter has been processed -post_daughter => sub { print "post_daughter: ", shift->get_name, "\n" }, # is executed if node has no daughter -no_daughter => sub { print "no_daughter: ", shift->get_name, "\n" }, # is executed whether or not node has sisters, if it does have sisters # they're processed first -in => sub { print "in: ", shift->get_name, "\n" }, # is executed if node has a sister, before sister is processed -pre_sister => sub { print "pre_sister: ", shift->get_name, "\n" }, # is executed if node has a sister, after sister is processed -post_sister => sub { print "post_sister: ", shift->get_name, "\n" }, # is executed if node has no sister -no_sister => sub { print "no_sister: ", shift->get_name, "\n" }, # is executed last -post => sub { print "post: ", shift->get_name, "\n" }, # specifies traversal order, default 'ltr' means first_daugher -> next_sister # traversal, alternate value 'rtl' means last_daughter -> previous_sister traversal -order => 'ltr', # ltr = left-to-right, 'rtl' = right-to-left Comments: =cut #$tree->visit_depth_first( # '-pre' => sub { print "pre: ", shift->get_name, "\n" }, # '-pre_daughter' => sub { print "pre_daughter: ", shift->get_name, "\n" }, # '-post_daughter' => sub { print "post_daughter: ", shift->get_name, "\n" }, # '-in' => sub { print "in: ", shift->get_name, "\n" }, # '-pre_sister' => sub { print "pre_sister: ", shift->get_name, "\n" }, # '-post_sister' => sub { print "post_sister: ", shift->get_name, "\n" }, # '-post' => sub { print "post: ", shift->get_name, "\n" }, # '-order' => 'ltr', #); sub visit_depth_first { my $self = shift; my %args = looks_like_hash @_; if ( $args{'-order'} and $args{'-order'} =~ /^rtl$/i ) { $args{'-sister_method'} = 'get_previous_sister'; $args{'-daughter_method'} = 'get_last_daughter'; } else { $args{'-sister_method'} = 'get_next_sister'; $args{'-daughter_method'} = 'get_first_daughter'; } $self->_visit_depth_first(%args); return $self; } sub _visit_depth_first { my ( $node, %args ) = @_; my ( $daughter_method, $sister_method ) = @args{qw(-daughter_method -sister_method)}; $args{'-pre'}->($node) if $args{'-pre'}; if ( my $daughter = $node->$daughter_method ) { $args{'-pre_daughter'}->($node) if $args{'-pre_daughter'}; $daughter->_visit_depth_first(%args); $args{'-post_daughter'}->($node) if $args{'-post_daughter'}; } else { $args{'-no_daughter'}->($node) if $args{'-no_daughter'}; } $args{'-in'}->($node) if $args{'-in'}; if ( my $sister = $node->$sister_method ) { $args{'-pre_sister'}->($node) if $args{'-pre_sister'}; $sister->_visit_depth_first(%args); $args{'-post_sister'}->($node) if $args{'-post_sister'}; } else { $args{'-no_sister'}->($node) if $args{'-no_sister'}; } $args{'-post'}->($node) if $args{'-post'}; } =item visit_breadth_first() Visits nodes breadth first Type : Visitor method Title : visit_breadth_first Usage : $tree->visit_breadth_first( -pre => sub{ ... }, -post => sub { ... } ); Function: Visits nodes in a breadth first traversal, executes handlers Returns : $tree Args : Optional handlers in the order in which they would be executed on an internal node: # first event handler, is executed when node is reached in recursion -pre => sub { print "pre: ", shift->get_name, "\n" }, # is executed if node has a sister, before sister is processed -pre_sister => sub { print "pre_sister: ", shift->get_name, "\n" }, # is executed if node has a sister, after sister is processed -post_sister => sub { print "post_sister: ", shift->get_name, "\n" }, # is executed if node has no sister -no_sister => sub { print "no_sister: ", shift->get_name, "\n" }, # is executed whether or not node has sisters, if it does have sisters # they're processed first -in => sub { print "in: ", shift->get_name, "\n" }, # is executed if node has a daughter, but before that daughter is processed -pre_daughter => sub { print "pre_daughter: ", shift->get_name, "\n" }, # is executed if node has a daughter, after daughter has been processed -post_daughter => sub { print "post_daughter: ", shift->get_name, "\n" }, # is executed if node has no daughter -no_daughter => sub { print "no_daughter: ", shift->get_name, "\n" }, # is executed last -post => sub { print "post: ", shift->get_name, "\n" }, # specifies traversal order, default 'ltr' means first_daugher -> next_sister # traversal, alternate value 'rtl' means last_daughter -> previous_sister traversal -order => 'ltr', # ltr = left-to-right, 'rtl' = right-to-left Comments: =cut sub visit_breadth_first { my $self = shift; my %args = looks_like_hash @_; if ( $args{'-order'} and $args{'-order'} =~ /rtl/i ) { $args{'-sister_method'} = 'get_previous_sister'; $args{'-daughter_method'} = 'get_last_daughter'; } else { $args{'-sister_method'} = 'get_next_sister'; $args{'-daughter_method'} = 'get_first_daughter'; } $self->_visit_breadth_first(%args); return $self; } sub _visit_breadth_first { my ( $node, %args ) = @_; my ( $daughter_method, $sister_method ) = @args{qw(-daughter_method -sister_method)}; $args{'-pre'}->($node) if $args{'-pre'}; if ( my $sister = $node->$sister_method ) { $args{'-pre_sister'}->($node) if $args{'-pre_sister'}; $sister->_visit_breadth_first(%args); $args{'-post_sister'}->($node) if $args{'-post_sister'}; } else { $args{'-no_sister'}->($node) if $args{'-no_sister'}; } $args{'-in'}->($node) if $args{'-in'}; if ( my $daughter = $node->$daughter_method ) { $args{'-pre_daughter'}->($node) if $args{'-pre_daughter'}; $daughter->_visit_breadth_first(%args); $args{'-post_daughter'}->($node) if $args{'-post_daughter'}; } else { $args{'-no_daughter'}->($node) if $args{'-no_daughter'}; } $args{'-post'}->($node) if $args{'-post'}; } =item visit_level_order() Visits nodes in a level order traversal. Type : Visitor method Title : visit_level_order Usage : $tree->visit_level_order( sub{...} ); Function: Visits nodes in a level order traversal, executes sub Returns : $tree Args : A subroutine reference that operates on visited nodes. Comments: =cut sub visit_level_order { my ( $self, $sub ) = @_; if ( UNIVERSAL::isa( $sub, 'CODE' ) ) { my @queue = ($self); while (@queue) { my $node = shift @queue; $sub->($node); if ( my $children = $node->get_children ) { push @queue, @{$children}; } } } else { throw 'BadArgs' => "'$sub' not a CODE reference"; } return $self; } =back =head2 UTILITY METHODS =over =item clone() Clones invocant. Type : Utility method Title : clone Usage : my $clone = $object->clone; Function: Creates a copy of the invocant object. Returns : A copy of the invocant. Args : Optional: a hash of code references to override reflection-based getter/setter copying my $clone = $object->clone( 'set_forest' => sub { my ( $self, $clone ) = @_; for my $forest ( @{ $self->get_forests } ) { $clone->set_forest( $forest ); } }, 'set_matrix' => sub { my ( $self, $clone ) = @_; for my $matrix ( @{ $self->get_matrices } ) { $clone->set_matrix( $matrix ); } ); Comments: Cloning is currently experimental, use with caution. It works on the assumption that the output of get_foo called on the invocant is to be provided as argument to set_foo on the clone - such as $clone->set_name( $self->get_name ). Sometimes this doesn't work, for example where this symmetry doesn't exist, or where the return value of get_foo isn't valid input for set_foo. If such a copy fails, a warning is emitted. To make sure all relevant attributes are copied into the clone, additional code references can be provided, as in the example above. Typically, this is done by overrides of this method in child classes. =cut sub clone { my $self = shift; $logger->info("cloning $self"); my %subs = @_; # we'll clone relatives in the tree, so no raw copying $subs{'set_parent'} = sub {}; $subs{'set_first_daughter'} = sub {}; $subs{'set_last_daughter'} = sub {}; $subs{'set_next_sister'} = sub {}; $subs{'set_previous_sister'} = sub {}; $subs{'set_child'} = sub {}; $subs{'insert'} = sub {}; return $self->SUPER::clone(%subs); } =back =head2 SERIALIZERS =over =item to_json() Serializes object to JSON string Type : Serializer Title : to_json() Usage : print $obj->to_json(); Function: Serializes object to JSON string Returns : String Args : None Comments: =cut sub to_json { my $node = shift; my %args = @_; my $extra_attr = \%args; $extra_attr->{'get_branch_length'} = 'length'; if ( my @children = @{ $node->get_children } ) { return '{' . $node->_to_json( $extra_attr ) . ',"children":[' . ( join ',', map { $_->to_json } @children ) . ']}'; } else { return '{' . $node->_to_json($extra_attr) . '}'; } } =item to_xml() Serializes invocant to xml. Type : Serializer Title : to_xml Usage : my $xml = $obj->to_xml; Function: Turns the invocant object (and its descendants )into an XML string. Returns : SCALAR Args : NONE =cut sub to_xml { my $self = shift; my @nodes = ( $self, @{ $self->get_descendants } ); my $xml = ''; # first write out the node elements for my $node ( @nodes ) { if ( my $taxon = $node->get_taxon ) { $node->set_attributes( 'otu' => $taxon->get_xml_id ); } if ( $node->is_root ) { $node->set_attributes( 'root' => 'true' ); } $xml .= "\n" . $node->get_xml_tag(1); } # then the rootedge? if ( my $length = shift(@nodes)->get_branch_length ) { my $target = $self->get_xml_id; my $id = "edge" . $self->get_id; $xml .= "\n" . sprintf('', $target, $id, $length); } # then the subtended edges for my $node ( @nodes ) { my $source = $node->get_parent->get_xml_id; my $target = $node->get_xml_id; my $id = "edge" . $node->get_id; my $length = $node->get_branch_length; if ( defined $length ) { $xml .= "\n" . sprintf('', $source, $target, $id, $length); } else { $xml .= "\n" . sprintf('', $source, $target, $id); } } return $xml; } =item to_newick() Serializes subtree subtended by invocant to newick string. Type : Serializer Title : to_newick Usage : my $newick = $obj->to_newick; Function: Turns the invocant object into a newick string. Returns : SCALAR Args : takes same arguments as Bio::Phylo::Unparsers::Newick Comments: takes same arguments as Bio::Phylo::Unparsers::Newick =cut { my ( $root_id, $string ); #no warnings 'uninitialized'; sub to_newick { my $node = shift; my %args = @_; $root_id = $node->get_id if not $root_id; my $blformat = '%f'; # first create the name my $name; if ( $node->is_terminal or $args{'-nodelabels'} ) { if ( not $args{'-tipnames'} ) { $name = $node->get_name; } elsif ( $args{'-tipnames'} =~ /^internal$/i ) { $name = $node->get_internal_name; } elsif ( $args{'-tipnames'} =~ /^taxon/i and $node->get_taxon ) { if ( $args{'-tipnames'} =~ /^taxon_internal$/i ) { $name = $node->get_taxon->get_internal_name; } elsif ( $args{'-tipnames'} =~ /^taxon$/i ) { $name = $node->get_taxon->get_name; } } else { $name = $node->get_generic( $args{'-tipnames'} ); } if ( $args{'-translate'} and exists $args{'-translate'}->{$name} ) { $name = $args{'-translate'}->{$name}; } } # now format branch length my $branch_length; if ( defined( $branch_length = $node->get_branch_length ) ) { if ( $args{'-blformat'} ) { $blformat = $args{'-blformat'}; } $branch_length = sprintf $blformat, $branch_length; } # now format nhx my $nhx; if ( $args{'-nhxkeys'} ) { my $sep; if ( $args{'-nhxstyle'} =~ /^mesquite$/i ) { $sep = ','; $nhx = '[%'; } else { $sep = ':'; $nhx = '[&&NHX:'; } my @nhx; for my $i ( 0 .. $#{ $args{'-nhxkeys'} } ) { my $key = $args{'-nhxkeys'}->[$i]; my $value = $node->get_generic($key); push @nhx, " $key = $value " if $value; } if (@nhx) { $nhx .= join $sep, @nhx; $nhx .= ']'; } else { $nhx = ''; } } # recurse further if ( my $first_daughter = $node->get_first_daughter ) { $string .= '('; $first_daughter->to_newick(%args); } # append to growing newick string $string .= ')' if $node->get_first_daughter; $string .= $name if defined $name; $string .= ':' . $branch_length if defined $branch_length; $string .= $nhx if $nhx; if ( $root_id == $node->get_id ) { undef $root_id; my $result = $string . ';'; undef $string; return $result; } # recurse further elsif ( my $next_sister = $node->get_next_sister ) { $string .= ','; $next_sister->to_newick(%args); } } } =begin comment Type : Internal method Title : _cleanup Usage : $trees->_cleanup; Function: Called during object destruction, for cleanup of instance data Returns : Args : =end comment =cut sub _cleanup { my $self = shift; my $id = $self->get_id; for my $field (@fields) { delete $field->{$id}; } } =begin comment Type : Internal method Title : _type Usage : $node->_type; Function: Returns : CONSTANT Args : =end comment =cut sub _type { $TYPE_CONSTANT } =begin comment Type : Internal method Title : _container Usage : $node->_container; Function: Returns : CONSTANT Args : =end comment =cut sub _container { $CONTAINER_CONSTANT } =back =cut # podinherit_insert_token # podinherit_start_token_do_not_remove # AUTOGENERATED pod created by /Users/rvosa/Applications/podinherit on Wed Mar 4 17:13:38 2009 # DO NOT EDIT the code below, rerun /Users/rvosa/Applications/podinherit instead. =pod =head1 INHERITED METHODS Bio::Phylo::Forest::Node inherits from one or more superclasses. This means that objects of class Bio::Phylo::Forest::Node also "do" the methods from the superclasses in addition to the ones implemented in this class. Below is the documentation for those additional methods, organized by superclass. =head2 SUPERCLASS Bio::Phylo::Taxa::TaxonLinker Bio::Phylo::Forest::Node inherits from superclass L. Below are the public methods (if any) from this superclass. =over =item get_taxon() Retrieves the Bio::Phylo::Taxa::Taxon object linked to the invocant. Type : Accessor Title : get_taxon Usage : my $taxon = $obj->get_taxon; Function: Retrieves the Bio::Phylo::Taxa::Taxon object linked to the invocant. Returns : Bio::Phylo::Taxa::Taxon Args : NONE Comments: =item set_taxon() Links the invocant object to a taxon object. Type : Mutator Title : set_taxon Usage : $obj->set_taxon( $taxon ); Function: Links the invocant object to a taxon object. Returns : Modified $obj Args : A Bio::Phylo::Taxa::Taxon object. =item unset_taxon() Unlinks the invocant object from any taxon object. Type : Mutator Title : unset_taxon Usage : $obj->unset_taxon(); Function: Unlinks the invocant object from any taxon object. Returns : Modified $obj Args : NONE =back =head2 SUPERCLASS Bio::Phylo::Listable Bio::Phylo::Forest::Node inherits from superclass L. Below are the public methods (if any) from this superclass. =over =item add_set() Type : Mutator Title : add_set Usage : $obj->add_set($set) Function: Associates a Bio::Phylo::Set object with the invocant Returns : Invocant Args : A Bio::Phylo::Set object =item add_to_set() Type : Mutator Title : add_to_set Usage : $listable->add_to_set($obj,$set); Function: Adds first argument to the second argument Returns : Invocant Args : $obj - an object to add to $set $set - the Bio::Phylo::Set object to add to Notes : this method assumes that $obj is already part of the invocant. If that assumption is violated a warning message is printed. =item can_contain() Tests if argument can be inserted in invocant. Type : Test Title : can_contain Usage : &do_something if $listable->can_contain( $obj ); Function: Tests if $obj can be inserted in $listable Returns : BOOL Args : An $obj to test =item clear() Empties container object. Type : Object method Title : clear Usage : $obj->clear(); Function: Clears the container. Returns : A Bio::Phylo::Listable object. Args : Note. Note : =item clone() Clones invocant. Type : Utility method Title : clone Usage : my $clone = $object->clone; Function: Creates a copy of the invocant object. Returns : A copy of the invocant. Args : None. Comments: Cloning is currently experimental, use with caution. =item contains() Tests whether the invocant object contains the argument object. Type : Test Title : contains Usage : if ( $obj->contains( $other_obj ) ) { # do something } Function: Tests whether the invocant object contains the argument object Returns : BOOLEAN Args : A Bio::Phylo::* object =item cross_reference() The cross_reference method links node and datum objects to the taxa they apply to. After crossreferencing a matrix with a taxa object, every datum object has a reference to a taxon object stored in its C<$datum-Eget_taxon> field, and every taxon object has a list of references to datum objects stored in its C<$taxon-Eget_data> field. Type : Generic method Title : cross_reference Usage : $obj->cross_reference($taxa); Function: Crossreferences the entities in the invocant with names in $taxa Returns : string Args : A Bio::Phylo::Taxa object Comments: =item current() Returns the current focal element of the listable object. Type : Iterator Title : current Usage : my $current_obj = $obj->current; Function: Retrieves the current focal entity in the invocant. Returns : A Bio::Phylo::* object Args : none. =item current_index() Returns the current internal index of the invocant. Type : Generic query Title : current_index Usage : my $last_index = $obj->current_index; Function: Returns the current internal index of the invocant. Returns : An integer Args : none. =item delete() Deletes argument from invocant object. Type : Object method Title : delete Usage : $obj->delete($other_obj); Function: Deletes an object from its container. Returns : A Bio::Phylo::Listable object. Args : A Bio::Phylo::* object. Note : Be careful with this method: deleting a node from a tree like this will result in undefined references in its neighbouring nodes. Its children will have their parent reference become undef (instead of pointing to their grandparent, as collapsing a node would do). The same is true for taxon objects that reference datum objects: if the datum object is deleted from a matrix (say), the taxon will now hold undefined references. =item first() Jumps to the first element contained by the listable object. Type : Iterator Title : first Usage : my $first_obj = $obj->first; Function: Retrieves the first entity in the invocant. Returns : A Bio::Phylo::* object Args : none. =item get_by_index() Gets element defined by argument index from invocant container. Type : Query Title : get_by_index Usage : my $contained_obj = $obj->get_by_index($i); Function: Retrieves the i'th entity from a listable object. Returns : An entity stored by a listable object (or array ref for slices). Args : An index or range. This works the way you dereference any perl array including through slices, i.e. $obj->get_by_index(0 .. 10)> $obj->get_by_index(0, -1) and so on. Comments: Throws if out-of-bounds =item get_by_name() Gets first element that has argument name Type : Visitor predicate Title : get_by_name Usage : my $found = $obj->get_by_name('foo'); Function: Retrieves the first contained object in the current Bio::Phylo::Listable object whose name is 'foo' Returns : A Bio::Phylo::* object. Args : A name (string) =item get_by_regular_expression() Gets elements that match regular expression from invocant container. Type : Visitor predicate Title : get_by_regular_expression Usage : my @objects = @{ $obj->get_by_regular_expression( -value => $method, -match => $re ) }; Function: Retrieves the data in the current Bio::Phylo::Listable object whose $method output matches $re Returns : A list of Bio::Phylo::* objects. Args : -value => any of the string datum props (e.g. 'get_type') -match => a compiled regular expression (e.g. qr/^[D|R]NA$/) =item get_by_value() Gets elements that meet numerical rule from invocant container. Type : Visitor predicate Title : get_by_value Usage : my @objects = @{ $obj->get_by_value( -value => $method, -ge => $number ) }; Function: Iterates through all objects contained by $obj and returns those for which the output of $method (e.g. get_tree_length) is less than (-lt), less than or equal to (-le), equal to (-eq), greater than or equal to (-ge), or greater than (-gt) $number. Returns : A reference to an array of objects Args : -value => any of the numerical obj data (e.g. tree length) -lt => less than -le => less than or equals -eq => equals -ge => greater than or equals -gt => greater than =item get_entities() Returns a reference to an array of objects contained by the listable object. Type : Generic query Title : get_entities Usage : my @entities = @{ $obj->get_entities }; Function: Retrieves all entities in the invocant. Returns : A reference to a list of Bio::Phylo::* objects. Args : none. =item get_index_of() Returns the index of the argument in the list, or undef if the list doesn't contain the argument Type : Generic query Title : get_index_of Usage : my $i = $listable->get_index_of($obj) Function: Returns the index of the argument in the list, or undef if the list doesn't contain the argument Returns : An index or undef Args : A contained object =item get_logger() Gets a logger object. Type : Accessor Title : get_logger Usage : my $logger = $obj->get_logger; Function: Returns a Bio::Phylo::Util::Logger object Returns : Bio::Phylo::Util::Logger Args : None =item get_sets() Type : Accessor Title : get_sets Usage : my @sets = @{ $obj->get_sets() }; Function: Retrieves all associated Bio::Phylo::Set objects Returns : Invocant Args : None =item insert() Pushes an object into its container. Type : Object method Title : insert Usage : $obj->insert($other_obj); Function: Pushes an object into its container. Returns : A Bio::Phylo::Listable object. Args : A Bio::Phylo::* object. =item insert_at_index() Inserts argument object in invocant container at argument index. Type : Object method Title : insert_at_index Usage : $obj->insert_at_index($other_obj, $i); Function: Inserts $other_obj at index $i in container $obj Returns : A Bio::Phylo::Listable object. Args : A Bio::Phylo::* object. =item is_in_set() Type : Test Title : is_in_set Usage : @do_something if $listable->is_in_set($obj,$set); Function: Returns whether or not the first argument is listed in the second argument Returns : Boolean Args : $obj - an object that may, or may not be in $set $set - the Bio::Phylo::Set object to query Notes : This method makes two assumptions: i) the $set object is associated with the invocant, i.e. add_set($set) has been called previously ii) the $obj object is part of the invocant If either assumption is violated a warning message is printed. =item last() Jumps to the last element contained by the listable object. Type : Iterator Title : last Usage : my $last_obj = $obj->last; Function: Retrieves the last entity in the invocant. Returns : A Bio::Phylo::* object Args : none. =item last_index() Returns the highest valid index of the invocant. Type : Generic query Title : last_index Usage : my $last_index = $obj->last_index; Function: Returns the highest valid index of the invocant. Returns : An integer Args : none. =item next() Returns the next focal element of the listable object. Type : Iterator Title : next Usage : my $next_obj = $obj->next; Function: Retrieves the next focal entity in the invocant. Returns : A Bio::Phylo::* object Args : none. =item notify_listeners() Notifies listeners of changed contents. Type : Utility method Title : notify_listeners Usage : $object->notify_listeners; Function: Notifies listeners of changed contents. Returns : Invocant. Args : NONE. Comments: =item previous() Returns the previous element of the listable object. Type : Iterator Title : previous Usage : my $previous_obj = $obj->previous; Function: Retrieves the previous focal entity in the invocant. Returns : A Bio::Phylo::* object Args : none. =item remove_from_set() Type : Mutator Title : remove_from_set Usage : $listable->remove_from_set($obj,$set); Function: Removes first argument from the second argument Returns : Invocant Args : $obj - an object to remove from $set $set - the Bio::Phylo::Set object to remove from Notes : this method assumes that $obj is already part of the invocant. If that assumption is violated a warning message is printed. =item remove_set() Type : Mutator Title : remove_set Usage : $obj->remove_set($set) Function: Removes association between a Bio::Phylo::Set object and the invocant Returns : Invocant Args : A Bio::Phylo::Set object =item set_listener() Attaches a listener (code ref) which is executed when contents change. Type : Utility method Title : set_listener Usage : $object->set_listener( sub { my $object = shift; } ); Function: Attaches a listener (code ref) which is executed when contents change. Returns : Invocant. Args : A code reference. Comments: When executed, the code reference will receive $object (the invocant) as its first argument. =item visit() Iterates over objects contained by invocant, executes argument code reference on each. Type : Visitor predicate Title : visit Usage : $obj->visit( sub{ print $_[0]->get_name, "\n" } ); Function: Implements visitor pattern using code reference. Returns : The invocant, possibly modified. Args : a CODE reference. =back =head2 SUPERCLASS Bio::Phylo::Util::XMLWritable Bio::Phylo::Forest::Node inherits from superclass L. Below are the public methods (if any) from this superclass. =over =item add_dictionary() Type : Mutator Title : add_dictionary Usage : $obj->add_dictionary($dict); Function: Adds a dictionary attachment to the object Returns : $self Args : Bio::Phylo::Dictionary =item get_attributes() Retrieves attributes for the element. Type : Accessor Title : get_attributes Usage : my %attrs = %{ $obj->get_attributes }; Function: Gets the xml attributes for the object; Returns : A hash reference Args : None. Comments: throws ObjectMismatch if no linked taxa object can be found =item get_dictionaries() Retrieves the dictionaries for the element. Type : Accessor Title : get_dictionaries Usage : my @dicts = @{ $obj->get_dictionaries }; Function: Retrieves the dictionaries for the element. Returns : An array ref of Bio::Phylo::Dictionary objects Args : None. =item get_namespaces() Type : Accessor Title : get_namespaces Usage : my %ns = %{ $obj->get_namespaces }; Function: Retrieves the known namespaces Returns : A hash of prefix/namespace key/value pairs, or a single namespace if a single, optional prefix was provided as argument Args : Optional - a namespace prefix =item get_tag() Retrieves tag name for the element. Type : Accessor Title : get_tag Usage : my $tag = $obj->get_tag; Function: Gets the xml tag name for the object; Returns : A tag name Args : None. =item get_xml_id() Retrieves xml id for the element. Type : Accessor Title : get_xml_id Usage : my $id = $obj->get_xml_id; Function: Gets the xml id for the object; Returns : An xml id Args : None. =item get_xml_tag() Retrieves tag string Type : Accessor Title : get_xml_tag Usage : my $str = $obj->get_xml_tag; Function: Gets the xml tag for the object; Returns : A tag, i.e. pointy brackets Args : Optional: a true value, to close an empty tag =item is_identifiable() By default, all XMLWritable objects are identifiable when serialized, i.e. they have a unique id attribute. However, in some cases a serialized object may not have an id attribute (governed by the nexml schema). This method indicates whether that is the case. Type : Test Title : is_identifiable Usage : if ( $obj->is_identifiable ) { ... } Function: Indicates whether IDs are generated Returns : BOOLEAN Args : NONE =item remove_dictionary() Type : Mutator Title : remove_dictionary Usage : $obj->remove_dictionary($dict); Function: Removes a dictionary attachment from the object Returns : $self Args : Bio::Phylo::Dictionary =item set_attributes() Assigns attributes for the element. Type : Mutator Title : set_attributes Usage : $obj->set_attributes( 'foo' => 'bar' ) Function: Sets the xml attributes for the object; Returns : $self Args : key/value pairs or a hash ref =item set_identifiable() By default, all XMLWritable objects are identifiable when serialized, i.e. they have a unique id attribute. However, in some cases a serialized object may not have an id attribute (governed by the nexml schema). For such objects, id generation can be explicitly disabled using this method. Typically, this is done internally - you will probably never use this method. Type : Mutator Title : set_identifiable Usage : $obj->set_tag(0); Function: Enables/disables id generation Returns : $self Args : BOOLEAN =item set_namespaces() Type : Mutator Title : set_namespaces Usage : $obj->set_namespaces( 'dwc' => 'http://www.namespaceTBD.org/darwin2' ); Function: Adds one or more prefix/namespace pairs Returns : $self Args : One or more prefix/namespace pairs, as even-sized list, or as a hash reference, i.e.: $obj->set_namespaces( 'dwc' => 'http://www.namespaceTBD.org/darwin2' ); or $obj->set_namespaces( { 'dwc' => 'http://www.namespaceTBD.org/darwin2' } ); Notes : This is a global for the XMLWritable class, so that in a recursive to_xml call the outermost element contains the namespace definitions. This method can also be called as a static class method, i.e. Bio::Phylo::Util::XMLWritable->set_namespaces( 'dwc' => 'http://www.namespaceTBD.org/darwin2'); =item set_tag() This method is usually only used internally, to define or alter the name of the tag into which the object is serialized. For example, for a Bio::Phylo::Forest::Node object, this method would be called with the 'node' argument, so that the object is serialized into an xml element structure called Type : Mutator Title : set_tag Usage : $obj->set_tag('node'); Function: Sets the tag name Returns : $self Args : A tag name (must be a valid xml element name) =item set_xml_id() This method is usually only used internally, to store the xml id of an object as it is parsed out of a nexml file - this is for the purpose of round-tripping nexml info sets. Type : Mutator Title : set_xml_id Usage : $obj->set_xml_id('node345'); Function: Sets the xml id Returns : $self Args : An xml id (must be a valid xml NCName) =item to_xml() Serializes invocant to XML. Type : XML serializer Title : to_xml Usage : my $xml = $obj->to_xml; Function: Serializes $obj to xml Returns : An xml string Args : None =back =head2 SUPERCLASS Bio::Phylo Bio::Phylo::Forest::Node inherits from superclass L. Below are the public methods (if any) from this superclass. =over =item clone() Clones invocant. Type : Utility method Title : clone Usage : my $clone = $object->clone; Function: Creates a copy of the invocant object. Returns : A copy of the invocant. Args : None. Comments: Cloning is currently experimental, use with caution. =item get() Attempts to execute argument string as method on invocant. Type : Accessor Title : get Usage : my $treename = $tree->get('get_name'); Function: Alternative syntax for safely accessing any of the object data; useful for interpolating runtime $vars. Returns : (context dependent) Args : a SCALAR variable, e.g. $var = 'get_name'; =item get_desc() Gets invocant description. Type : Accessor Title : get_desc Usage : my $desc = $obj->get_desc; Function: Returns the object's description (if any). Returns : A string Args : None =item get_generic() Gets generic hashref or hash value(s). Type : Accessor Title : get_generic Usage : my $value = $obj->get_generic($key); or my %hash = %{ $obj->get_generic() }; Function: Returns the object's generic data. If an argument is used, it is considered a key for which the associated value is returned. Without arguments, a reference to the whole hash is returned. Returns : A string or hash reference. Args : None =item get_id() Gets invocant's UID. Type : Accessor Title : get_id Usage : my $id = $obj->get_id; Function: Returns the object's unique ID Returns : INT Args : None =item get_internal_name() Gets invocant's 'fallback' name (possibly autogenerated). Type : Accessor Title : get_internal_name Usage : my $name = $obj->get_internal_name; Function: Returns the object's name (if none was set, the name is a combination of the $obj's class and its UID). Returns : A string Args : None =item get_logger() Gets a logger object. Type : Accessor Title : get_logger Usage : my $logger = $obj->get_logger; Function: Returns a Bio::Phylo::Util::Logger object Returns : Bio::Phylo::Util::Logger Args : None =item get_name() Gets invocant's name. Type : Accessor Title : get_name Usage : my $name = $obj->get_name; Function: Returns the object's name. Returns : A string Args : None =item get_obj_by_id() Attempts to fetch an in-memory object by its UID Type : Accessor Title : get_obj_by_id Usage : my $obj = Bio::Phylo->get_obj_by_id($uid); Function: Fetches an object from the IDPool cache Returns : A Bio::Phylo object Args : A unique id =item get_score() Gets invocant's score. Type : Accessor Title : get_score Usage : my $score = $obj->get_score; Function: Returns the object's numerical score (if any). Returns : A number Args : None =item new() The Bio::Phylo root constructor, is rarely used directly. Rather, many other objects in Bio::Phylo internally go up the inheritance tree to this constructor. The arguments shown here can therefore also be passed to any of the child classes' constructors, which will pass them on up the inheritance tree. Generally, constructors in Bio::Phylo subclasses can process as arguments all methods that have set_* in their names. The arguments are named for the methods, but "set_" has been replaced with a dash "-", e.g. the method "set_name" becomes the argument "-name" in the constructor. Type : Constructor Title : new Usage : my $phylo = Bio::Phylo->new; Function: Instantiates Bio::Phylo object Returns : a Bio::Phylo object Args : Optional, any number of setters. For example, Bio::Phylo->new( -name => $name ) will call set_name( $name ) internally =item set_desc() Sets invocant description. Type : Mutator Title : set_desc Usage : $obj->set_desc($desc); Function: Assigns an object's description. Returns : Modified object. Args : Argument must be a string. =item set_generic() Sets generic key/value pair(s). Type : Mutator Title : set_generic Usage : $obj->set_generic( %generic ); Function: Assigns generic key/value pairs to the invocant. Returns : Modified object. Args : Valid arguments constitute: * key/value pairs, for example: $obj->set_generic( '-lnl' => 0.87565 ); * or a hash ref, for example: $obj->set_generic( { '-lnl' => 0.87565 } ); * or nothing, to reset the stored hash, e.g. $obj->set_generic( ); =item set_name() Sets invocant name. Type : Mutator Title : set_name Usage : $obj->set_name($name); Function: Assigns an object's name. Returns : Modified object. Args : Argument must be a string, will be single quoted if it contains [;|,|:\(|\)] or spaces. Preceding and trailing spaces will be removed. =item set_score() Sets invocant score. Type : Mutator Title : set_score Usage : $obj->set_score($score); Function: Assigns an object's numerical score. Returns : Modified object. Args : Argument must be any of perl's number formats, or undefined to reset score. =item to_json() Serializes object to JSON string Type : Serializer Title : to_json() Usage : print $obj->to_json(); Function: Serializes object to JSON string Returns : String Args : None Comments: =item to_string() Serializes object to general purpose string Type : Serializer Title : to_string() Usage : print $obj->to_string(); Function: Serializes object to general purpose string Returns : String Args : None Comments: This is YAML =back =cut # podinherit_stop_token_do_not_remove =head1 SEE ALSO =over =item L This object inherits from L, so methods defined there are also applicable here. =item L This object inherits from L, so methods defined there are also applicable here. =item L Also see the manual: L and L. =back =head1 REVISION $Id: Node.pm 844 2009-03-05 00:07:26Z rvos $ =cut } 1; __DATA__ sub add_Descendent{ my ( $self,$child ) = @_; $self->set_child( $child ); return scalar @{ $self->get_children }; } sub each_Descendent{ my $self = shift; if ( my $children = $self->get_children ) { return @{ $children }; } return; } sub get_all_Descendents{ my $self = shift; if ( my $desc = $self->get_descendants ) { return @{ $desc }; } return; } *get_Descendents = \&get_all_Descendents; *is_Leaf = \&is_terminal; *is_otu = \&is_terminal; sub descendent_count{ my $self = shift; my $count = 0; if ( my $desc = get_descendants ) { $count = scalar @{ $desc }; } return $count; } sub height{ shift->calc_max_path_to_tips } sub depth{ shift->calc_path_to_root } sub branch_length{ my $self = shift; if ( @_ ) { $self->set_branch_length(shift); } return $self->get_branch_length; } sub id { my $self = shift; if ( @_ ) { $self->set_name(shift); } return $self->get_name; } sub internal_id { shift->get_id } sub description { my $self = shift; if ( @_ ) { $self->set_desc(shift); } return $self->get_desc; } sub bootstrap { my ( $self, $bs ) = @_; if ( defined $bs && looks_like_number $bs ) { $self->set_score($bs); } return $self->get_score; } sub ancestor { my $self = shift; if ( @_ ) { $self->set_parent(shift); } return $self->get_parent; } sub invalidate_height { } sub add_tag_value{ my $self = shift; if ( @_ ) { my ( $key, $value ) = @_; $self->set_generic( $key, $value ); } return 1; } sub remove_tag { my ( $self, $tag ) = @_; my %hash = %{ $self->get_generic }; my $exists = exists $hash{$tag}; delete $hash{$tag}; $self->set_generic(); $self->set_generic(%hash); return !!$exists; } sub remove_all_tags{ shift->set_generic() } sub get_all_tags { my $self = shift; my %hash = %{ $self->get_generic }; return keys %hash; } sub get_tag_values{ my ( $self, $tag ) = @_; my $values = $self->get_generic($tag); return ref $values ? @{ $values } : $values; } sub has_tag{ my ( $self, $tag ) = @_; my %hash = %{ $self->get_generic }; return exists $hash{$tag}; } sub id_output { shift->get_internal_name }